Insertion sequence fingerprinting and transpositonal activity in mycobacteria

by N"Dingsa G. Fomukong

Written in English
Published: Downloads: 50
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Edition Notes

StatementN"Dingsa G. Fomukong.
ContributionsUniversity of Surrey. School of Biological Sciences.
ID Numbers
Open LibraryOL19354640M

Identification of non-tuberculous mycobacteria NTM have become relevant after the beginning of the Aids pandemic (9). The morbidity and mortality impact of NTM in HIV-positive patients stimulated studies on epidemiology, ecology, genetics and molecular biology of mycobacteria. The recent increase in occurrence of mycobacterioses also led to. Related Pages. On This Site Tests: AFB Smear and Culture, Susceptibility Testing Conditions: HIV/AIDS, Tuberculosis, Lung Diseases. Elsewhere On The Web Lung Foundation Australia: NonTuberculous Mycobacteria (NTM) MedlinePlus: Mycobacterial Infections American Lung Association: Learn about Nontuberculous Mycobacteria (NTM) NTM Info and Research. T1 - Standard operating procedure for optimal identification of mycobacteria using 16S rRNA gene sequences. AU - Tortoli, Enrico. PY - Y1 - N2 - Genetic sequencing is increasingly used for the identification of bacteria and for many of them, including mycobacteria, the analysis of the 16S rRNA gene represents the gold by: We use/store this info to ensure you have proper access and that your account is secure. We may use this info to send you notifications about your account, your institutional acce.

  Mycobacteria other than M. bovis may interfere with current bovine tuberculosis diagnostic tests resulting in false positive test results. As the prevalence of M. bovis decreases in the United States, interference from other mycobacteria play an increasingly important role in preventing the eradication of M. bovis. To identify mycobacteria other than M. bovis that may be interfering with Cited by:   The insertion sequence (IS) is specific for the Mycobacterium tuberculosis complex (MTBC) []. ulosis strains typically contain multiple copies of IS (up to 25 per genome) [], although strains with only a single copy or no copies have also been identified [3–5].In contrast, strains are characterized by having a low copy number, with by: Laboratory Detection and Identification of Mycobacteria, 2nd Edition View Sample Pages This guideline provides recommendations for medical mycobacteriology laboratories on the optimal approach for diagnosis of mycobacterial infections. Our authors and editors. We are a community of more than , authors and editors from 3, institutions spanning countries, including Nobel Prize winners and some of Cited by: 8.

in bacteria evolution. Here, we report the complete sequence of a , bp circular plasmid (pCBMA_2) from an atypical and fast-growing environmental mycobacteria. This is a unique plasmid, in comparison with the characterised mycobacteria plasmids, harboring a type IV-like and ESX-P2 type VII secretion systems. pCBMA_2 can be furtherCited by: 1. Optimal identification. of mycobacteria Standards in Genomic Sciences. nique, limited at first to highly specialized labora-tories, has become in recent years very popular mainly due to the robustness of the chemistry (based on the Big Dye Terminator technology) and to the excellent performance of modern automatic instrumentations. The mycobacteria: an introduction to nomenclature and pathogenesis.   Classification of Mycobacteria by HPLC and Pattern Recognition Abstract Mycobacteria include a number of respiratory and non-respiratory pathogens for humans, such as M. tuberculosis, the causative agent of the disease for which it is named. Identification of the responsible.

Insertion sequence fingerprinting and transpositonal activity in mycobacteria by N"Dingsa G. Fomukong Download PDF EPUB FB2

Mycobacteria differ so strongly from other bacteria in their cell wall architecture and metabolism that they require specific diagnostic tests, i.e.

stains, culture media, identification methods. Tuberculosis and leprosy are well-known diseases of mankind, but Buruli ulcer disease and nontuberculous mycobacterial diseases are gaining recognition as important diseases in specific settings.

The sequence of three independent copies have been determined and these were virtually identical, differing in only a few base pairs.

1°,12,13 The number of IS copies and the sites of insertion in the chromosome are highly variable from strain to strain, indicating that Cited by: Cirillo Insertion sequence fingerprinting and transpositonal activity in mycobacteria book, Barletta RG, Bloom BR, Jacobs WR., Jr A novel transposon trap for mycobacteria: isolation and characterization of IS J Bacteriol.

Dec; (24)– [PMC free article] Collins DM, Stephens DM. Identification of an insertion sequence, IS, in Mycobacterium bovis. FEMS Microbiol Lett. Sep 15; 67 (1)–Cited by: An insertion sequence-like element, IS, was isolated from a Mycobacterium tuberculosis cosmid library as a repetitive sequence.

IS shows similarities with elements of the IS3 family. This insertion sequence was found to be specific to mycobacteria belonging to the M. tuberculosis by: V 57 12 tinental flight. Non-tuberculous mycobacteria can be transmitted in raw milk or insufficiently heat-treated meat.

Water and soil are frequent sources of mycobacterial infections either in the form of direct contacts for aquarists or gardeners or by means of aerosols in showers or indoor swimming by: For rapidly growing mycobacteria (RGM), the KDa heat shock protein and RNA polymerase Beta subunit genes are more variable than the 16S rRNA gene, making these targets useful in the discrimiation of closely related species such as M.

abscessus, M. Isolation of mycobacteria and their identification based on phenotypical manifestations, particular-ly culture, has been constantly used as a “golden standard” (Taylor et al., ). Due to the fact that these methods are much more time consuming, their use is on the decline.

They have been replaced by methods based on DNA detection. In tuberculosis, it is often important to establish the source of infection and to determine whether disease is due to a new strain of Mycobacterium tuberculosis or to relapse.

To cope with the resurgence of tuberculosis and atypical mycobacterioses in AIDS patients, on the one hand, and to overcome the limitations of classical bacteriological procedures on the other, the development of rapid Cited by: Semi, H., Bottger, E C, and VilJanen, M K () Identification of mycobacteria by PCR-based sequence determination of a kilodalton protein gene J Clin Mxrob – Google Scholar by:   Summary.

An insertion sequence, IS, in the genome of Mycobacterium bovis has been identified and sequenced. It is bp long with 15 bp inverted repeat ends and contains a large ORF.

There are six copies of IS in the genome of M. bovis and the element is also present in Mycobacterium is not closely related to other DNA elements described in Cited by: Katoch V M. DNA fingerprinting of Mycobacterium tuberculosis isolates from Agra region by is probe.

Indian J Med Microbiol [serial online] [cited Oct 7 ]; Mycobacterium is a genus of Actinobacteria, given its own family, the species are recognized in this genus. This genus includes pathogens known to cause serious diseases in mammals, including tuberculosis (Mycobacterium tuberculosis) and leprosy (Mycobacterium leprae) in humans.

The Greek prefix myco-means "fungus," alluding to the way mycobacteria have been Domain: Bacteria. “Molecular Genetics of Mycobacteria is an extraordinary book. It includes chapters from virtually every major scientific contributor to the genetic understanding of mycobacteria.

For anyone entering the field or seeking to learn about fundamental genetic and molecular aspects of mycobacteria, this book 5/5(2).

The Ziehl-Neelsen staining for the direct detection of mycobacteria by microscopy is used to identify acid fast bacilli. The lipid rich cell wall of mycobacteria makes it resistant to Gram stain. It can also be used to stain few other bacteria like Nocardia. The reagents used for the staining are carbolfuchsin, acid-alcohol and methylene blue.

Different subtypes of Mycobacterium tuberculosis (MTB) may induce diverse severe human infections, and some of their symptoms are similar to other pathogenes, e.g. Nontuberculosis mycobacteria (NTM). So determination of mycobacterium subtypes facilitates the effective control of MTB infection and proliferation.

This study exploits a novel DNA barcoding visualization method for Cited by: 2. INTRODUCTION. Mycobacterial species other than Mycobacterium tuberculosis and Mycobacterium leprae are generally free-living organisms that are ubiquitous in the environment.

They have been recovered from water, soil, domestic and wild animals, milk, and food [].As the incidence of tuberculosis (TB) declined in areas of the world where socioeconomic conditions were rapidly advancing, the. Nucleotide sequence accession number. The GenBank. ac-cession number for the nucleotide sequence of insertion element.

IS is M UNIV OF NEBRASKA-LINCOLN on September 5, Downloaded from. Mycobacteria AEROBIC, non-motile, rod-shaped Gram-positive BACTERIA (see GRAM'S STAIN), some of which are found in are important PATHOGENS of man and ANIMALS, for example Mycobacterium leprae, which is the causative agent of LEPROSY, and Mycobacterium tuberculosis which is the causative agent of name ‘Mycobacterium’ derives from the occasional.

The prevalence of lung disease due to infections with nontuberculous mycobacteria (NTM) has been increasing and surpassed tuberculosis (TB) in some countries. Treatment outcomes are often unsatisfactory, highlighting an urgent need for new anti-NTM medications. Although NTM in general do not respond well to TB specific drugs, the similarities between NTM and Mycobacterium Cited by: Nucleic acid sequence based amplification (NASBA) for the identification of Mycobacteria Article (PDF Available) in Journal of general microbiology (10) November with 95 Reads.

identification of mycobacteria. The goal of this module is to discuss the classical and emerging methods for identification of mycobacteria, advantages and limitations of the different methods, importance of quality, accurate identification results, clinical significance of mycobacteria, turnaround times and reporting considerations,File Size: 2MB.

This technique is particularly useful to identify mycobacteria species for which nucleic acid probes are unavailable, including rapid-growing mycobacteria species of the M. fortuitum-chelonae group.

The technique requires isolation of bacterial DNA, amplification, sequencing, and data analysis. PCR Identification of Mycobacteria 10thNational Conference on Laboratory Aspects of Tuberculosis Ap Ryan Jepson Microbiology Supervisor State Hygienic Laboratory at the University of Iowa.

60 other mycobacteria and related species that produce mycolic acids, such as Corynebacteria, Dietzia, Gordonia, Nocardia, Rhodococcus and Tsukamurella. The Sherlock MYCO-LCS was created by microbiologists for microbiologists, so no HPLC experience is necessary.

Start studying Microbiology Horizontal Gene Transfer. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Insertion sequence. Found at either end of transposable element. Same sequence on both sides but facing different directions (inverted).

Transposon. contain additional genes between two insertion. Procedures for the Isolation and Identification of Mycobacteria ( edition) on *FREE* shipping on qualifying offers. A novel transposon trap for mycobacteria: Isolation and characterization of IS Journal of bacteriology, (24), This insertion element exhibits several characteristics that suggest it may be a useful tool for genetic analysis of mycobacteria, possibly including the study of mechanisms of pathogenesis.", Cited by: species of mycobacteria including M.

tuberculosis, respectively. The GenoType MTBC is based on a 23S rRNA gene fragment specific for the M. tuberculosis complex, together with gyrB sequence polymorphisms, and the RD1 deletion for identification of M.

bovis BCG. Several ‘in. Mycobacteria Identification System Operating Manual (Version B) MIDI, Inc. Sandy Drive Newark, DE USA June Tel: () The mycobacteria: an introduction to nomenclature and pathogenesis N.

Rastogi, E. Legrand & C. Sola Unité de la Tuberculose et des Mycobactéries, Institut Pasteur, B.P.Pointe-à-Pitre Cedex, Guadeloupe Summary Tuberculosis, caused by Mycobacterium tuberculosis, and leprosy, caused by M.

leprae, are diseases known since antiquity File Size: 3MB. () Occurrence and stability of of insertion sequences in Mycobacterium tuberculosis complex strains: evaluation of an insertion sequence-dependent DNA polymorphism as a tool in the epidemiology of tuberculosis. J Clin Microbiol – .Cited by:   Molecular identification of Mycobacterium species has two primary advantages when compared to phenotypic identification: rapid turn-around time and improved accuracy.

The information content of the 5' end of the 16S ribosomal RNA gene (16S rDNA) is sufficient for identification of most bacterial species. However, reliable sequence-based identification is hampered by many faulty and Cited by: MYCS Mycobacteria DNA Sequencing GA Test Code Method DNA Sequencing This assay is routinely ordered as a reflex from test # or to identify the atypical species (e.g.

Mycobacterium gordonae) present in the patient sample. Specimens Bronchial Washings: (min ) mL, ambient (24 hrs) or refrigerated (7 days), in sterile plastic leak-proof Size: KB.